Characterization of the hrpZ gene from Pseudomonas syringae pv. maculicolaM2

Revista de microbiologia

Valenzuela-Soto, Humberto¹  Marsch, Rodolfo²  Rodríguez-Ríos, Dalia³  Álvarez-Mejía, César⁴  López-Ramírez, Varinia⁵  Hernández-Guzmán, Gustavo⁶ 

[1] Centro de Investigación en Química Aplicada, Coahuila, MX;Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, D.F. México, MX;Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Guanajuato, MX;Instituto Tecnológico Superior de Irapuato Plantel Abasolo, Guanajuato, MX;Instituto Tecnológico Superior de Irapuato, Guanajuato, MX;Universidad de Guanajuato, Guanajuato, MX

关键词: hrpZ;    mutant non-pathogenic;    transmid;    Tn5;    phylogenetic.;   

DOI  :  10.1590/S1517-838246320140655

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemented with a crude extract from the leaves of Arabidopsis thaliana. A mutant containing a transposon insertion in the hrpZ gene (PsmMut8) was unable to infect adult plants from Arabidopsis thaliana or Brassica oleracea, suggesting a loss of pathogenicity. The promotorless cat reporter present in the gene trap was expressed if PsmMut8 was grown in minimal medium (M9) supplemented with the leaf extract but not if grown in normal rich medium (KB). We conducted phylogenetic analysis using hrpAZB genes, showing the classical 5-clade distribution, and nucleotide diversity analysis, showing the putative position for selective pressure in this operon. Our results indicate that the hrpAZB operon from Pseudomonas syringaepv. maculicola M2 is necessary for its pathogenicity and that its diversity would be under host-mediated diversifying selection.

【 授权许可】

CC BY-NC