Morphological and Biological Evaluations of Human Periodontal Ligament Fibroblasts in Contact with Different Bovine Bone Grafts Treated with Low-Temperature Deproteinisation Protocol

International Journal of Molecular Sciences

Giuseppe Varvara¹  Serena Bianchi²  Antonella Mattei²  Loredana Cristiano²  Guido Macchiarelli²  Enrico Marchetti²  Diana Torge²  Sara Bernardi²  Leonardo Mancini² 

[1] Department of Innovative Technologies in Medicine and Dentistry, University of Chieti-Pescara ‘Gabriele d’Annunzio’, Via Dei Vestini 11, 66100 Chieti, Italy;Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy;

关键词: biomaterial;    cell culture;    deproteinised bovine bone;    fibroblasts;    periodontal regeneration;   

DOI  :  10.3390/ijms23095273

来源: DOAJ

【 摘 要 】

Several types of deproteinised bovine bone mineral (DBBM) are available on the market, and each one is obtained with a thermic and chemical process that can differ, achieving different results. Currently, several protocols using low temperature are suggested to reduce the possible particle crystallisation during the production process. This study aimed to evaluate the biomorphological reaction of periodontal fibroblast cultures in contact with different DBBM particles treated with a low-temperature protocol (Thermagen^®) and without exposure to sodium hydroxide (NaOH). Morphological evaluation was performed using light, confocal laser, and scanning electron microscopy, and the biological reaction in terms of proliferation was performed using an XTT proliferation assay at 24 h (T1), 72 h (T2), and 7 days (T3). The morphological analysis highlighted how the presence of the materials stimulated a change in the morphology of the cells into a polygonal shape, surface reactions with the thickening of the membrane, and expression of actin. In particular, the morphological changes were appreciable from T1, with a progressive increase in the considered morphological characteristics at T2 and T3 follow-ups. The proliferation assay showed a statistical significance between the different experimental materials and the negative control in T2 and T3 follow-ups. The post hoc analysis did not reveal any differences between the materials. In conclusion, the grafts obtained with the low-temperature extractions protocol and not exposed to NaOH solution showed positive morphological reactions with no differences in the sizes of particles.

【 授权许可】

Unknown