Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth

Genetics and Molecular Biology

Fernanda Timm Seabra Souza¹  Alexandre Silva De Mello¹  Kristiane Michelin¹  Janice Carneiro Coelho¹ 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Bioquímica Serviço de Genética Médica Hospital de Clínicas de Porto AlegrePorto Alegre RS ,Brazil

关键词: amikacin;    cephalothin;    clindamycin;    vancomycin;    fibroblasts;    cell culture;   

DOI  :  10.1590/S1415-47572004000300023

来源: SciELO

【 摘 要 】

The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.

【 授权许可】

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