Chitosan-Based Anti-Oxidation Delivery Nano-Platform: Applications in the Encapsulation of DHA-Enriched Fish Oil

Marine Drugs

Chwen-Jen Shieh¹  Chien-Liang Lee²  Chitsan Lin³  Ming-Fong Tsai⁴  Chia-Hung Kuo⁴  Po-Kai Chang⁴  Chun-Yung Huang⁴ 

[1] Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan;Department of Chemical and Materials Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan;Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan;Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan;

关键词: powdered fish oil;    docosahexaenoic acid;    chitosan nanoparticles;    encapsulation efficiency;    loading capacity;    TGA;   

DOI  :  10.3390/md19080470

来源: DOAJ

【 摘 要 】

Refined cobia liver oil is a nutritional supplement (CBLO) that is rich in polyunsaturated fatty acids (PUFAs), such as DHA and EPA; however, PUFAs are prone to oxidation. In this study, the fabrication of chitosan-TPP-encapsulated CBLO nanoparticles (CS@CBLO NPs) was achieved by a two-step method, including emulsification and the ionic gelation of chitosan with sodium tripolyphosphate (TPP). The obtained nanoparticles were inspected by dynamic light scattering (DLS) and showed a positively charged surface with a z-average diameter of between 174 and 456 nm. Thermogravimetric analysis (TGA) results showed the three-stage weight loss trends contributing to the water evaporation, chitosan decomposition, and CBLO decomposition. The loading capacity (LC) and encapsulation efficiency (EE) of the CBLO loading in CS@CBLO NPs were 17.77–33.43% and 25.93–50.27%, respectively. The successful encapsulation of CBLO in CS@CBLO NPs was also confirmed by the Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) techniques. The oxidative stability of CBLO and CS@CBLO NPs was monitored by FTIR. As compared to CBLO, CS@CBLO NPs showed less oxidation with a lower generation of hydroperoxides and secondary oxidation products after four weeks of storage. CS@CBLO NPs are composed of two ingredients that are beneficial for health, chitosan and fish oil in a nano powdered fish oil form, with an excellent oxidative stability that will enhance its usage in the functional food and pharmaceutical industries.

【 授权许可】

Unknown