A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion

Bioengineering

Yi-Chin Toh¹  Danny van Noort²  Anju Raja³  Hanry Yu³ 

[1] Department of Biomedical Engineering, 4 Engineering Drive, National University of Singapore, Singapore 117853, Singapore;Division of Biotechnology, IFM, Linköping University, Linköping 58183, Sweden;Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, 04-01, 31 Biopolis Way, Singapore 138669, Singapore;

关键词: 3D cell culture;    microfluidics;    cell migration;    cell invasion;    metastasis;   

DOI  :  10.3390/bioengineering5020029

来源: DOAJ

【 摘 要 】

We have developed a microfluidic-based culture chip to simulate cancer cell migration and invasion across the basement membrane. In this microfluidic chip, a 3D microenvironment is engineered to culture metastatic breast cancer cells (MX1) in a 3D tumor model. A chemo-attractant was incorporated to stimulate motility across the membrane. We validated the usefulness of the chip by tracking the motilities of the cancer cells in the system, showing them to be migrating or invading (akin to metastasis). It is shown that our system can monitor cell migration in real time, as compare to Boyden chambers, for example. Thus, the chip will be of interest to the drug-screening community as it can potentially be used to monitor the behavior of cancer cell motility, and, therefore, metastasis, in the presence of anti-cancer drugs.

【 授权许可】

Unknown