New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes

BMC Genomics

He Zhang¹  Xun Xu¹  An Xiao¹  Huanming Yang¹  Guangyi Fan¹  Xin Liu¹  Eugenia Boulygina²  Svetlana Tsygankova²  Konstantin V. Gunbin³  Dmitry A. Afonnikov³  Mariya Y. Pakharukova³  Mikhail A. Genaev³  Viatcheslav A. Mordvinov³  Kirill Ustyantsev³  Nikita I. Ershov³  Alexander G. Blinov³  Nikolay Chekanov⁴  Konstantin G. Skryabin⁴  Alexander Mazur⁴  Egor B. Prokhortchouk⁴  Victor Solovyev⁵  Simon Ming-Yuen Lee⁶  Ekaterina Khrameeva⁷ 

[1] BGI-Shenzhen;Federal Research Center Kurchatov Institute;Institute of Cytology and Genetics SB RAS;Russian Federal Research Center for Biotechnology;Softberry Inc.;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau;ZAO Genoanalytica;

关键词: Opisthorchiidae;    Opisthorchis felineus;    Genome;    Trans-splicing;    Microintrons;    Liver flukes;   

DOI  :  10.1186/s12864-019-5752-8

来源: DOAJ

【 摘 要 】

Abstract Background The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease. Results Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis. We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis. We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes. Conclusion All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases.

【 授权许可】

Unknown