Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration

Thoracic Cancer

Shuping Yang¹  Jiaqi Ji²  Yongwen Li³  Ying Li³  Lingling Zu³ 

[1] Department of Anesthesiology Huashan Hospital Affiliated to Fudan University Shanghai China;Department of Pulmonary and Critical Care Medicine Sichuan Academy of Medical Sciences & Sichuan Province People's Hospital Chengdu China;Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute Tianjin Medical University General Hospital Tianjin China;

关键词: deubiquitylating enzyme;    invasion;    migration;    non‐small cell lung cancer;    USP41;   

DOI  :  10.1111/1759-7714.13843

来源: DOAJ

【 摘 要 】

Abstract Background To reveal the function of deubiquitylating enzyme USP41 in lung adenocarcinoma. Methods The relationship between USP41 and lung cancer was determined by analyzing data from The Cancer Genome Atlas (TCGA). A549 and H1299 cell lines were transfected with short hairpin RNA against USP41 (shUSP41 group) or negative control (shCon group). Western blotting was used to verify the transfection efficacy and marker expression. Cell proliferation and apoptosis were analyzed by EdU assay, MTT assay, and flow cytometry after USP41 knockdown. Transwell assay was used to determine the effect of USP41 downregulation on cell migration. Results Analysis of lung cancer data from TCGA database indicated a higher level of USP41 expression in lung cancer tumor tissue compared with that in noncancerous tissue, and USP41 overexpression was correlated with poor overall survival of lung cancer patients (p < 0.01). The outcomes of the EdU, MTT, and flow cytometry assays indicated decreased cell proliferation and enhanced apoptosis in shUSP41‐transfected cells. Transwell assay further demonstrated that USP41 knockdown increased the migration rate of A549 and H1299 cells. Conclusions In our study, USP41 was overexpressed in lung cancer tissue and associated with poor prognosis of lung cancer. USP41 knockdown inhibits cell proliferation and migration and induces cell apoptosis of lung cancer.

【 授权许可】

Unknown