Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

Applied Sciences

Małgorzata Daczewska¹  Magda Dubińska-Magiera¹  Jolanta Saczko²  Nina Rembiałkowska²  Julita Kulbacka²  Anna Szewczyk²  Andrzej Sikora³  Wojciech Szlasa⁴  Hanna Czapor-Irzabek⁵ 

[1] Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, 50-137 Wroclaw, Poland;Department of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, 50-367 Wroclaw, Poland;Department of Nanometrology, Faculty of Microsystem Electronics and Photonics,Wroclaw, University of Science and Technology, Janiszewskiego 11/17, 50-372 Wroclaw, Poland;Faculty of Medicine, Wroclaw Medical University, J. Mikulicza-Radeckiego Str., 50-367 Wroclaw, Poland;Laboratory of Elemental Analysis and Structural Research, Wroclaw Medical University, 50-367 Wroclaw, Poland;

关键词: electroporation;    electrochemotherapy;    breast cancer;    electropermeabilization;    doxorubicin;    MCF−7/WT;   

DOI  :  10.3390/app10082765

来源: DOAJ

【 摘 要 】

Electroporation increases the transmembrane transport of molecules. The combination of electric pulses with cytostatic compounds is beneficial for cancer treatment. Doxorubicin (DOX) is a commonly used chemotherapeutic anticancer drug. Its fluorescence properties enable the investigation of drug distribution and metabolism. In this study, doxorubicin was enhanced by electroporation to eliminate cancer cells more effectively. The influence of electroporation on the drug uptake was evaluated in two cell lines: MCF-7/WT and MCF-7/DOX. The intracellular localization of doxorubicin and its impact on the intracellular structure organization were examined under a confocal microscope. Cellular effects were examined with the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test that estimates the rate of metabolism in viable cells. The ultrastructure (TEM) of tumor cells subjected to the electric field was analyzed. An enhanced doxorubicin efficacy was observed in MCF-7/DOX cells after combination with electroporation. The response of the resistant cell line was revealed to be more sensitive to electric pulses. Electroporation-based methods may be attractive for cancer treatment in human breast adenocarcinoma, especially with acquired resistance. Electroporation enables a reduction of the effective dose of the drugs and the exposure time in this type of cancer, diminishing side effects of the systemic therapy.

【 授权许可】

Unknown