Respiratory Syncytial Virus (RSV) G Protein Vaccines With Central Conserved Domain Mutations Induce CX3C-CX3CR1 Blocking Antibodies

Viruses

RebeccaM. DuBois¹  AnaM. Nuñez Castrejon¹  HarrisonC. Bergeron²  Jackelyn Murray²  RalphA. Tripp² 

[1] Department of Biomolecular Engineering, University of California-Santa Cruz, Santa Cruz, CA 95064, USA;Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA;

关键词: respiratory syncytial virus;    RSV;    vaccine;    G protein;    G glycoprotein;    antibodies;   

DOI  :  10.3390/v13020352

来源: DOAJ

【 摘 要 】

Respiratory syncytial virus (RSV) infection can cause bronchiolitis, pneumonia, morbidity, and some mortality, primarily in infants and the elderly, for which no vaccine is available. The RSV attachment (G) protein contains a central conserved domain (CCD) with a CX3C motif implicated in the induction of protective antibodies, thus vaccine candidates containing the G protein are of interest. This study determined if mutations in the G protein CCD would mediate immunogenicity while inducing G protein CX3C-CX3CR1 blocking antibodies. BALB/c mice were vaccinated with structurally-guided, rationally designed G proteins with CCD mutations. The results show that these G protein immunogens induce a substantial anti-G protein antibody response, and using serum IgG from the vaccinated mice, these antibodies are capable of blocking the RSV G protein CX3C-CX3CR1 binding while not interfering with CX3CL1, fractalkine.

【 授权许可】

Unknown