PEGylation of Superparamagnetic Iron Oxide Nanoparticles with Self-Organizing Polyacrylate-PEG Brushes for Contrast Enhancement in MRI Diagnosis

Nanomaterials

Etelka Tombácz¹  Imre Földesi²  Katalin Farkas²  IldikóY. Tóth³  Erzsébet Illés³  Márta Szekeres³  Ákos Szabó⁴  Béla Iván⁴ 

[1] Department of Food Engineering, University of Szeged, Moszkvai krt. 5-7, H-6725 Szeged, Hungary;Department of Laboratory Medicine, University of Szeged, Semmelweis u. 6, H-6720 Szeged, Hungary;Department of Physical Chemistry and Materials Science, University of Szeged, Aradi Vt. 1, H-6720 Szeged, Hungary;Polymer Chemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, P.O. Box 286, H-1519 Budapest, Hungary;

关键词: superparamagnetic nanoparticles;    PEG coating;    core–shell nanoparticles;    blood compatibility;    colloidal stability;    MRI contrast agents;   

DOI  :  10.3390/nano8100776

来源: DOAJ

【 摘 要 】

For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate-co-acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Illés et al. J. Magn. Magn. Mater. 2018, 451, 710–720). In the present work, we elaborated on the molecular background of the formation of the P(PEGMA-AA)-coated MNPs, and of their remarkable colloidal stability and salt tolerance by using potentiometric acid–base titration, adsorption isotherm determination, infrared spectroscopy (FT-IR ATR), dynamic light scattering, and electrokinetic potential determination methods. The P(PEGMA-AA)@MNPs have excellent blood compatibility as demonstrated in blood sedimentation, smears, and white blood cell viability experiments. In addition, blood serum proteins formed a protein corona, protecting the particles against aggregation (found in dynamic light scattering and electrokinetic potential measurements). Our novel particles also proved to be promising candidates for MRI diagnosis, exhibiting one of the highest values of r2 relaxivity (451 mM−1s−1) found in literature.

【 授权许可】

Unknown