Understanding the interactions between porphyrin-containing photosensitizers and polymer-coated nanoparticles in model biological environments

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:461

Article

Jenkins, Samir V.¹  Srivatsan, Avinash²  Reynolds, Kimberly Y.¹  Gao, Feng¹  Zhang, Yongbin³  Heyes, Colin D.¹  Pandey, Ravindra K.²  Chen, Jingyi¹ 

[1] Univ Arkansas, Dept Chem & Biochem, Fayetteville, AR 72701 USA

[2] Roswell Pk Canc Inst, Photodynam Therapy Ctr, Buffalo, NY 14263 USA

[3] US FDA, NCTR ORA Nanotechnol Core Facil, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

关键词: Gold nanostructure;    Drug delivery;    PEG coating;    Controlled release;   

DOI  :  10.1016/j.jcis.2015.09.037

来源: Elsevier

【 摘 要 】

Non-covalent incorporation of hydrophobic drugs into polymeric systems is a commonly-used strategy for drug delivery because non-covalent interactions minimize modification of the drug molecules whose efficacy is retained upon release. The behaviors of the drug-polymer delivery system in the biological environments it encounters will affect the efficacy of treatment. In this report, we have investigated the interaction between a hydrophobic drug and its encapsulating polymer in model biological environments using a photosensitizer encapsulated in a polymer-coated nanoparticle system. The photosensitizer, 3-(1'-hexyloxyethyl)-3-devinylpyropheophorbide-a (HPPH), was non-covalently incorporated to the poly(ethylene glycol) (PEG) layer coated on Au nanocages (AuNCs) to yield AuNC-HPPH complexes. The non-covalent binding was characterized by Scatchard analysis, fluorescence lifetime, and Raman experiments. The dissociation constant between PEG and HPPH was found to be similar to 35 mu M with a maximum loading of similar to 2.5 x 10(5) HPPHs/AuNC. The release was studied in serum-mimetic environment and in vesicles that model human cell membranes. The rate of protein-mediated drug release decreased when using a negatively-charged or cross-linked terminus of the surface-modified PEG. Furthermore, the photothermal effect of AuNCs can initiate burst release, and thus allow control of the release kinetics, demonstrating on-demand drug release. This study provides insights regarding the actions and release kinetics of non-covalent drug delivery systems in biological environments. (C) 2015 Elsevier Inc. All rights reserved.

【 授权许可】

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