Screening of six medicinal plant species for antileishmanial activity

Acta Pharmaceutica

Demarchi Izabel Galhardo¹  Junior Ranulfo Piau²  Fernandez Carla Maria Mariano³  Gazim Zilda Cristiani³  Silveira Thaís Gomes Verzignassi⁴  Ferreira Fabiana Borges Padilha⁴  Lonardoni Maria Valdrinez Campana⁴  Cabral Márcia Regina Pereira⁵  Sarragiotto Maria Helena⁶ 

[1] Federal University of Santa Catarina, Florianópolis – Santa Catarina State, Brazil;Posgraduate Program in Animal Science of the University of Paranaense, Universidade Paranaense UNIPAR, Umuarama – Paraná State, Brazil;Posgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Umuarama – Paraná State, Brazil;Posgraduate Program in Health Sciences Maringá State University, , Brazil;Posgraduate Program in Pharmaceutical Sciences, Maringá State University, Maringá – Paraná State, Brazil;Postgraduate Program in Chemistry, Maringá State University, Maringá – Paraná, Brazil;

关键词: piper corcovadensis;    hymenaea stigonocarpa;    antileishmanial;    leishmania amazonensis;    leishmania braziliensis;    cytotoxicity;    hemolysis;   

DOI  :  10.2478/acph-2021-0023

来源: DOAJ

【 摘 要 】

This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %.

【 授权许可】

Unknown