UPLC-MS/MS Phytochemical Analysis of Two Croatian Cistus Species and Their Biological Activity

Life

Olivera Politeo¹  Mila Radan¹  Ivana Carev¹  Zoran Zorić²  Nada Ilić³  Ana Maravić³  Vedrana Čikeš Čulić⁴ 

[1] Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia;Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia;Faculty of Science, University of Split, 21000 Split, Croatia;School of Medicine, University of Split, 21000 Split, Croatia;

关键词: antimicrobial;    antioxidant;    antiproliferative;    Cistus;    flavonoid;    UPLC-MS/MS;   

DOI  :  10.3390/life10070112

来源: DOAJ

【 摘 要 】

Aqueous extracts of two Cistus species wild growing in Croatia—Cistus creticus (CC) and Cistus salviifolius (CS)—have been assessed with UPLC-MS/MS, showing 43 different phytochemicals, with flavonol glycosides: myricetin-3-hexoside and myricetin-rhamnoside, predominate ones in CC and myricetin-3-hexoside in CS. Antioxidant potential tested with the FRAP method showed no difference between CS and CC aqueous extracts, while higher phenolic content of CC comparing to CS, determined with a Folin–Cicolateu reagent correlated to its higher antioxidant capacity observed by the DPPH method. Both extracts were assessed for antimicrobial activity, using disc-diffusion and broth microdilution assays, targeting the opportunistic pathogens, associated with food poisoning, urinary, respiratory tract, blood stream and wound infections in humans. Antimicrobial assays revealed that fungi were in general more sensitive to both Cistus aqueous extracts, comparing to the bacteria where two extracts showed very similar activity. The most potent activity was observed against A. baumannii for both extracts. The extracts were tested on human lung cancer (A549) cell line using the MTT assay, showing very similar antiproliferative activity. After 72 h treatment with CC and CS aqueous extracts in concentration of 0.5 g/L, the viability of the cells were 37% and 50% respectively, compared to non-treated cells.

【 授权许可】

Unknown