Biomolecules | |
Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome | |
RékaEszter Sziva1  Dóra Gerszi1  Krisztina Lajtai1  Szabolcs Várbíró1  Zoltán Giricz2  Péter Ferdinandy2  CsillaTerézia Nagy2  GyörgyLászló Nádasy3  Róbert Tarszabó3  EszterMária Horváth3  Rita Benkő3  Bálint Bányai3  Leila Hadjadj4  | |
[1] Department of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, Hungary;Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary;Department of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary;Institute of Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary; | |
关键词: hyperandrogenism; vitamin D; polycystic ovary syndrome (PCOS); insulin resistance; autophagy; oxidative stress; | |
DOI : 10.3390/biom9090471 | |
来源: DOAJ |
【 摘 要 】
Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D−T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T−) and vitamin D deficient animals (D−T−) served as controls. (N = 10−12 per group). Results: D−T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D−T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D−T− rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.
【 授权许可】
Unknown