Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome

Biomolecules

RékaEszter Sziva¹  Dóra Gerszi¹  Krisztina Lajtai¹  Szabolcs Várbíró¹  Zoltán Giricz²  Péter Ferdinandy²  CsillaTerézia Nagy²  GyörgyLászló Nádasy³  Róbert Tarszabó³  EszterMária Horváth³  Rita Benkő³  Bálint Bányai³  Leila Hadjadj⁴ 

[1] Department of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, Hungary;Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary;Department of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary;Institute of Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary;

关键词: hyperandrogenism;    vitamin D;    polycystic ovary syndrome (PCOS);    insulin resistance;    autophagy;    oxidative stress;   

DOI  :  10.3390/biom9090471

来源: DOAJ

【 摘 要 】

Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D−T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T−) and vitamin D deficient animals (D−T−) served as controls. (N = 10−12 per group). Results: D−T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D−T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D−T− rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.

【 授权许可】

Unknown