Production of carotenoid sarcinaxanthin by Kocuria palustris isolated from Northeastern Brazil Caatinga soil and their antioxidant and photoprotective activities

Electronic Journal of Biotechnology

Esteban Espinosa Vidal¹  Maria Betânia Melo de Oliveira²  Brígida Thais Luckwu de Lucena³  Paulo Vitor Dias Mendes⁴  Tayane de Cássia Dias Mendes-Silva⁵  Maria Tereza dos Santos Correia⁵  Márcia Vanusa da Silva⁵  Rosileide Fontenele da Silva Andrade⁶  Karine da Cunha Schmidt⁷  Raquel de Fátima Rodrigues de Souza⁷  Fernanda Granja da Silva Oliveira⁸ 

[1] Corresponding author.;Centro de Ciências Biológicas e Sociais Aplicadas, Universidade Estadual da Paraíba, Campus V, Brazil;Centro de Estudos e Pesquisas em Plantas Medicinais, Universidade Federal do Vale do São Francisco, Brazil;Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Brazil;Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Brazil;Departamento de Design, Centro de artes e comunicação, Universidade Federal de Pernambuco, Brazil;Grupo de Biotecnologia Branca, Laboratório de Bioprocessos, Centro de Tecnologias Estratégicas do Nordeste, Brazil;Núcleo de Pesquisas em Ciências Ambientais e Biotecnologia, Universidade Católica de Pernambuco, Brazil;

关键词: Antioxidant;    Bacteria;    Bioactive compound;    Factorial design;    Biological activities;    C50 carotenoid;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: This study aimed to produce carotenoids of two bacterial strains obtained and isolated from Caatinga soil in Northeastern Brazil and to evaluate their antioxidant and photoprotective activities. The morphological identification of bacteria was performed by Gram staining and molecularly confirmed through the 16S rRNA gene. The production of carotenoids was performed on two 23 factorial designs to analyze the influence of independent variables (temperature range, luminosity, agitation, spiral presence, and bacterial isolate type) for maximum carotenoid yield. The selected condition has been transferred to a bioreactor (10L). The identification of carotenoids was performed by liquid chromatography (HPLC) and mass spectrometry (LC-MS). Antioxidant activity was determined by inhibiting the β-carotene/linoleic acid system and the effectiveness as sunscreen was measured through its sun protection factor (SPF). Results: The results revealed that the isolates FT-7.22 and FT-5.12 were identified as Kocuria palustris; producers of a rare C50 carotenoid sarcinaxanthin. This is the first report on the production of carotenoids by this species from the Caatinga Domain. The pigment that was obtained from the Tryptic Soy Broth (TSB) medium in the best conditions of the factorial designs (increased agitation, aeration, and light exposure) exhibited a significant increase in the carotenoid production. The isolated FT-7.22 reached a higher sarcinaxanthin concentration (112,480 μg/L), and it exhibited promising antioxidant (76.53 ± 0.09%) and photoprotective activities (SPF = 9.36 ± 0.52). Conclusions: This study demonstrated the ability of K. palustris to produce carotenoid sarcinaxanthin with antioxidant and photoprotective activities so that it can be applied in cosmetic formulations.How to cite: Mendes-Silva TCD, Vidal EE, de Souza RFR, et al. Production of carotenoid sarcinaxanthin by Kocuria palustris isolated from Northeastern Brazil Caatinga soil and their antioxidant and photoprotective activities. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.05.004

【 授权许可】

Unknown