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Effect of ultrasound treatment on the extraction of antioxidants from Ardisia compressa Kunth fruits and identification of phytochemicals by HPLC-ESI-MS
Diana Beatriz Muñiz-Márquez1  Guillermo Cristian G. Martínez-Ávila2  Juan Alberto Ascacio-Valdés3  Romeo Rojas4  Jorge Enrique Wong-Paz4  Pedro Aguilar-Zárate4  Alma Yolanda Vázquez-Sánchez5 
[1] Corresponding author.;
[2] Autonomous University of Coahuila, School of Chemistry, Food Research Department, Boulevard Venustiano Carranza S/N, Colonia República Oriente, Saltillo, Coahuila, C.P. 25280, Mexico;
[3] Autonomous University of Nuevo Leon, School of Agronomy, Laboratory of Chemistry and Biochemistry, Francisco I. Madero S/N, Ex Hacienda el Cañada, General Escobedo, Nuevo León, C.P. 66050, Mexico;
[4] Tecnológico Nacional de México, Campus Ciudad Valles, Departamento de Ingenierías, Carretera al Ingenio Plan de Ayala Km. 2, Colonia Vista Hermosa, Ciudad Valles, San Luis Potosí, C.P. 79010, Mexico;
[5] Universidad Tecnológica de Xicotepec de Juárez Área Agroindustrial Alimentaria. Av, Universidad Tecnológica No. 1000, Tierra Negra, Xicotepec de Juárez, Puebla, C.P. 73080, Mexico;
关键词: Bioactive compound;    Antioxidant;    Phenolic compound;    Chemical composition of food;    Biochemical characterization of food;    Phenolic compounds;   
DOI  :  
来源: DOAJ
【 摘 要 】

The influence of ultrasound-assisted extraction of phytochemicals from Ardisia compressa Kunth on the antioxidant capacity was investigated. The factors evaluated were: ultrasound extraction time (10, 20 and 30 min), ethanol concentration (0, 35, 70 %) and solid/liquid ratio (1:4, 1:8 and 1:12 g mL−1). The L9 (3)3 array was applied, and the DPPH• scavenging capacity of treatments was evaluated to obtain optimal extraction conditions. Finally, the phytochemicals were characterized by high-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS). Ten minutes of ultrasound extraction using 0 % of ethanol and solid/liquid ratio 1:12 g mL−1 were the optimal conditions of extraction. The HPLC-ESI-MS analysis revealed the presence of gluconic acid, quercetin-3-O-glucoside, isorhamnetin-3-O-rutinoside, demethylligstroside, ponicidin, 4-caffeoylquinic acid, rosmarinic acid, and galloyl-hexoside. The optimal ultrasound-assisted extraction conditions were defined by applying the Taguchi methodology. The phytochemicals identified in A. compressa fruits suggest its use as a potential source of bioactive compounds.

【 授权许可】

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