Polyenoic acid metabolism in cultured dissociated brain cells

Journal of Lipid Research

John H. Menkes¹  Ephraim Yavin² 

[1] Brentwood VA Hospital, Los Angeles, California 90025;Division of Pediatric Neurology, University of California at Los Angeles, Los Angeles, California 90024 and;

关键词: linolenic acid;    fatty acids;    choline phosphoglyceride metabolism;    ethanolamine phosphoglyceride metabolism;    fatty acid chain elongation;    triglyceride biosynthesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

The incorporation of [1-14C]linolenate (18:3 n – 3) into cellular lipids of cultured dissociated brain cells was studied. During the initial phases of incubation, radioactivity was found in free fatty acids, diacylglycerols, triacylglycerols, and choline phosphoglyceride pools preferentially. Incorporation into the ethanolamine phosphoglyceride pool increased steadily and paralleled desaturation and chain elongation of 18:3 → 20:3 → 20:4 → 20:5 → 22:5 → 22:6. From pulse–chase studies it was evident that the label of the highly polyunsaturated fatty acids in ethanolamine phosphoglycerides is constantly increased while the label in the fatty acids of choline phosphoglycerides decreased. Uptake of 18:3 by the cells was reduced by lowering incubation temperature, the triacylglycerol and ethanolamine phosphoglyceride pools being mainly affected. Lowering the incubation temperatures essentially abolished conversion of labeled 18:3 to the higher polyenoic acids. At intermediate temperatures, conversion of 18:3 to 20:5 n – 3 was still active, but conversion of 20:5 n – 3 to 22:6 n – 3 was abolished, suggesting that formation of 22:6 from 18:3 proceeds by at least two reactions distinguishable by their temperature dependency.

【 授权许可】

Unknown