Full-Length Transcriptome Sequencing Reveals the Impact of Cold Stress on Alternative Splicing in Quinoa

International Journal of Molecular Sciences

Yuanyuan Li¹  Hao Li²  Hui Zhang²  Yanxiu Zhao²  Yiwu Zhao²  Shiqi Luo²  Ling Zheng²  Yifeng Gan²  Changle Ma²  Xiang Liu²  Qun Shao² 

[1] CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China;Shandong Provincial Key Laboratory of Plant Stress, Life Science College, Shandong Normal University, Jinan 250014, China;

关键词: quinoa;    full-length transcriptomes;    cold stress;    ROS homeostasis;   

DOI  :  10.3390/ijms23105724

来源: DOAJ

【 摘 要 】

Quinoa is a cold-resistant and nutrient-rich crop. To decipher the cold stress response of quinoa, the full-length transcriptomes of the cold-resistant quinoa variety CRQ64 and the cold-sensitive quinoa variety CSQ5 were compared. We identified 55,389 novel isoforms and 6432 novel genes in these transcriptomes. Under cold stress, CRQ64 had more differentially expressed genes (DEGs) and differentially alternative splicing events compared to non-stress conditions than CSQ5. DEGs that were specifically present only in CRQ64 were significantly enriched in processes which contribute to osmoregulation and ROS homeostasis in plants, such as sucrose metabolism and phenylpropanoid biosynthesis. More genes with differential alternative splicing under cold stress were enriched in peroxidase functions in CRQ64. In total, 5988 transcription factors and 2956 long non-coding RNAs (LncRNAs) were detected in this dataset. Many of these had altered expression patterns under cold stress compared to non-stress conditions. Our transcriptome results demonstrate that CRQ64 undergoes a wider stress response than CSQ5 under cold stress. Our results improved the annotation of the quinoa genome and provide new insight into the mechanisms of cold resistance in quinoa.

【 授权许可】

Unknown