Herding cats: Label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis

iScience

Keisuke Motone¹  Nicolas Cardozo¹  Jeff Nivala¹ 

[1] Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA;

关键词: Bioorganic chemistry;    Biochemistry;    Proteomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Proteins carry out life's essential functions. Comprehensive proteome analysis technologies are thus required for a full understanding of the operating principles of biological systems. While current proteomics techniques suffer from limitations in sensitivity and/or throughput, nanopore technology has the potential to enable de novo protein identification through single-molecule sequencing. However, a significant barrier to achieving this goal is controlling protein/peptide translocation through the nanopore sensor for processive strand analysis. Here, we review recent approaches that use a range of techniques, from oligonucleotide conjugation to molecular motors, aimed at driving protein strands and peptides through protein nanopores. We further discuss site-specific protein conjugation chemistry that could be combined with these translocation approaches as future directions to achieve single-molecule protein detection and sequencing of native proteins.

【 授权许可】

Unknown