Proteomic changes in various plant tissues associated with chromium stress in sunflower

Saudi Journal of Biological Sciences

Rana Khalid Iqbal¹  Jiri Krucky¹  Rahul Datta²  Shah Fahad³  Subhan Danish⁴  Anis Ali Shah⁵  Rehana Sardar⁶  Shakil Ahmed⁶  Asma Zulfiqar⁶  Umber Ghafoor⁷  Mohammad Javed Ansari⁸  Shabir Hussain⁹  Sami Al Obaid^1,10 

[1] Corresponding authors.;Department of Agronomy, The University of Haripur, Haripur 22620, Pakistan;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou 570228, China;Department of Agronomy, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan;Department of Botany, Division of Science and Technology University of Education, Lahore, 54770 Punjab, Pakistan;Department of Botany, University of the Punjab, Lahore, 54000 Punjab, Pakistan;Department of Soil Science, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60800 Punjab, Pakistan;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou 570228, China;Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60800 Punjab, Pakistan;Pesticide Residue Laboratory, Kala Shah Kaku, Punjab, Pakistan;

关键词: Chromium;    Heavy metal;    Helianthus annuus;    Proteomics;    Physiological alterations;   

DOI  :  

来源: DOAJ

【 摘 要 】

Heavy metal stress is one of the major abiotic stresses that cause environmental pollution in recent decades. An elevated concentration of these heavy metals is highly toxic to plant. Chromium (Cr) is one of the heavy metals whose concentration in the environment is still increasing alarmingly. It is harmful for plant growth and achene yield. To check out the growth and protein alternation towards pollutants, two sunflower varieties (RA-713 and AHO-33) were subjected to different chromium concentrations (control, 200 ppm, 400 ppm) by soil application. This study has elaborated that 400 ppm Cr resulted in a reduction of various growth parameters. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to enhance the understanding of plant proteomic modulation under Cr stress. Different protein bands like 48 and 49, 26 kDa have newly appeared, and three 60, 47, and 42 kDa, and two protein bands 49 and 13 kDa were up-regulated in seeds of RA-713 and AHO-33, respectively. Some proteins (52, 16 kDa) are down-regulated in leaf tissues of both varieties. Only 6 and 81 kDa protein showed up-regulation and 154 kDa down-regulation behavior in the shoot in response to stress. The finding s of study might support the selection of tolerant genotype under Cr contamination and the discovery of new protein biomarkers that can use as monitoring tools in heavy metal stress biology.

【 授权许可】

Unknown