In Vitro Inhibition of Zika Virus Replication with Amantadine and Rimantadine Hydrochlorides

Microbiology Research

Gilbert D. Loría¹  Eugenia Corrales-Aguilar¹  Jorge L. Arias-Arias²  Francisco Vega-Aguilar³  Dihalá Picado-Soto³ 

[1] Centro de Investigación en Enfermedades Tropicales (CIET), Universidad de Costa Rica, San José 11501-2060, Costa Rica;Sección de Biología Celular y Molecular, Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica;Sección de Virología, Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica;

关键词: Zika;    dengue;    yellow fever;    antivirals;    adamantanes;    amantadine;   

DOI  :  10.3390/microbiolres12030052

来源: DOAJ

【 摘 要 】

Zika virus (ZIKV) is a mosquito-borne flavivirus in which human infection became relevant during recent outbreaks in Latin America due to its unrecognized association with fetal neurological disorders. Currently, there are no approved effective antivirals or vaccines for the treatment or prevention of ZIKV infections. Amantadine and rimantadine are approved antivirals used against susceptible influenza A virus infections that have been shown to have antiviral activity against other viruses, such as dengue virus (DENV). Here, we report the in vitro effectiveness of both amantadine and rimantadine hydrochlorides against ZIKV replication, resulting in a dose-dependent reduction in viral titers of a ZIKV clinical isolate and two different ZIKV reference strains. Additionally, we demonstrate similar in vitro antiviral activity of these drugs against DENV-1 and yellow fever virus (YFV), although at higher drug concentrations for the latter. ZIKV replication was inhibited at drug concentrations well below cytotoxic levels of both compounds, as denoted by the high selectivity indexes obtained with the tested strains. Further work is absolutely needed to determine the potential clinical use of these antivirals against ZIKV infections, but our results suggest the existence of a highly conserved mechanism across flavivirus, susceptible to be blocked by modified more specific adamantane compounds.

【 授权许可】

Unknown