Membrane and envelope virus proteins co-expressed as lysosome associated membrane protein (LAMP) fused antigens: a potential tool to develop DNA vaccines against flaviviruses

Anais da Academia Brasileira de Ciências

Rafael Dhalia²  Milton Maciel Jr.¹  Fábia S.p. Cruz²  Isabelle F.t. Viana²  Mariana L. Palma²  Thomas August¹  Ernesto T.a. Marques Jr.² 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisas Aggeu Magalhães Departamento de Virologia Laboratório de Virologia e Terapia ExperimentalRecife PE ,Brasil

关键词: dengue;    West Nile;    yellow fever;    Lysosome-Associated Membrane Protein - LAMP;    DNA vaccines;    dengue;    febre do Oeste do Nilo;    febre amarela;    Proteína de Associação à Membrana Lisossomal - LAMP;    vacinas de DNA;   

DOI  :  10.1590/S0001-37652009000400005

来源: SciELO

【 摘 要 】

Vaccination is the most practical and cost-effective strategy to prevent the majority of the flavivirus infection to which there is an available vaccine. However, vaccines based on attenuated virus can potentially promote collateral side effects and even rare fatal reactions. Given this scenario, the developent of alternative vaccination strategies such as DNA-based vaccines encoding specific flavivirus sequences are being considered. Endogenous cytoplasmic antigens, characteristically plasmid DNA-vaccine encoded, are mainly presented to the immune system through Major Histocompatibility Complex class I - MHC I molecules. The MHC I presentation via is mostly associated with a cellular cytotoxic response and often do not elicit a satisfactory humoral response. One of the main strategies to target DNA-encoded antigens to the MHC II compartment is expressing the antigen within the Lysosome-Associated Membrane Protein (LAMP). The flavivirus envelope protein is recognized as the major virus surface protein and the main target for neutralizing antibodies. Different groups have demonstrated that co-expression of flavivirus membrane and envelope proteins in mammalian cells, fused with the carboxyl-terminal of LAMP, is able to induce satisfactory levels of neutralizing antibodies. Here we reviewed the use of the envelope flavivirus protein co-expression strategy as LAMP chimeras with the aim of developing DNA vaccines for dengue, West Nile and yellow fever viruses.

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