Effect of Grass Carp Scale Collagen Peptide FTGML on cAMP-PI3K/Akt and MAPK Signaling Pathways in B16F10 Melanoma Cells and Correlation between Anti-Melanin and Antioxidant Properties

Foods

Xiaomei Sha¹  Zongcai Tu²  Lu Zhang²  Zizi Hu²  Sheng Huang³ 

[1] College of Life Science, Jiangxi Normal University, Nanchang 330022, China;National R&D Center for Freshwater Fish Processing, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;

关键词: collagen peptide;    B16F10 cells;    melanogenesis;    tyrosinase;    antioxidant;   

DOI  :  10.3390/foods11030391

来源: DOAJ

【 摘 要 】

Peptide Phe-Thr-Gly-Met-Leu (FTGML) is a bioactive oligopeptide with tyrosinase inhibitory activity derived from gelatin hydrolysate of grass carp scales. Previous studies have shown that FTGML addition can effectively inhibit mushroom tyrosinase activity in vitro, and also has some effect on the inhibition of melanogenesis in zebrafish in vivo, but the underlying mechanism is not fully understood. In this study, we used FTGML to treat B16F10 melanoma cells, and found a significant inhibition of tyrosinase activity and melanin synthesis. Interestingly, the treatment showed a strong correlation between antioxidant activity and anti-melanin, which was associated with FTGML reducing the involvement of reactive oxygen species in melanin synthesis. Furthermore, FTGML reduced melanogenesis in B16F10 cells by downregulating the cAMP-PI3K/Akt and MAPK pathways (p38 and JNK). These results suggested that FTGML can reduce melanin production in mouse B16F10 melanoma cells through multiple pathways.

【 授权许可】

Unknown