Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine

Biomimetics

Vincent Ball¹  Salima El Yakhlifi¹  Ovidiu Ersen²  Dris Ihiawakrim² 

[1] Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 1121, 11 rue Humann, CEDEX, 67085 Strasbourg, France;Institut de Physique et de Chimie des Matériaux, UMR 7504 CNRS—Université de Strasbourg, 23 rue du Loess, BP 43, CEDEX 2, 67034 Strasbourg, France;

关键词: melanin-like nanoparticles;    sodium periodate;    enzymatic activity;    layer-by-layer films;   

DOI  :  10.3390/biomimetics3040036

来源: DOAJ

【 摘 要 】

Polydopamine (PDA) deposition, obtained from the oxidation of dopamine and other catecholamines, is a universal way to coat all known materials with a conformal coating which can subsequently be functionalized at will. The structural analogies between polydopamine and eumelanin, the black-brown pigment of the skin, were incited to produce stable polydopamine nanoparticles in solution, instead of amorphous precipitates obtained from the oxidation of dopamine. Herein, we demonstrate that size-controlled and colloidally stable PDA-based nanoparticles can be obtained in acidic conditions, where spontaneous auto-oxidation of dopamine is suppressed, using sodium periodate as the oxidant and a protein, like alkaline phosphatase (ALP), as a templating agent. The size of the PDA@ALP nanoparticles depends on the dopamine/enzyme ratio and the obtained particles display enzymatic activity of alkaline phosphatase, with an activity extending up to two weeks after particle synthesis. The PDA@ALP nanoparticles can be engineered in polyelectrolyte multilayered films to potentially design model biosensors.

【 授权许可】

Unknown