The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant

International Journal of Nanomedicine

关键词: titanium;    alkali-heat treatment;    magnetron sputtering;    osteogenic differentiation;    osteoclast inhibition;   

DOI  :  

来源: DOAJ

【 摘 要 】

Haiyan Wang, 1 Qiuping Xu, 1 Hui Hu, 2 Chunling Shi, 3 Ziyan Lin, 3 Huixi Jiang, 3 Huaipu Dong, 3 Jing Guo 11Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, 250012, People’s Republic of China;2Osaka Dental University Kusuha School, Hirakata City, Osaka 573-1121, Japan;3School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, People’s Republic of ChinaCorrespondence: Jing GuoDepartment of Orthodontics, Room 201, School and Hospital of Stomatology, Shandong University, 44-1 Wenhua West Road, Jinan, Shandong 250012, Mainland ChinaEmail [e-mail]: Relying on surface topography alone to enhance the osteointegration of implants is still inadequate. An effective way to combine long-term ion release and surface topography to enhance osteogenic property is urgently needed.Purpose: The objective of this study is to fabricate a long-term strontium ion release implant system and confirm the biological function in vitro and in vivo.Methods: The biomimic surface was fabricated through alkali-heat treatment and magnetron sputtering. The in vitro biological function assays were determined by MTT, fluorescence staining, alkaline phosphatase activity, extracellular mineralization, and quantitative real-time polymerase chain reaction assays. The in vivo experiments were detected by micro-CT, HE staining and Masson staining.Results: The biomimic surface structure has been successfully fabricated. The in vitro cell assays determined that AH-Ti/Sr90 possessed the best biological function. The in vivo experiments demonstrated that AH-Ti/Sr90 could promote osteointegration significantly under both in normal and osteoporotic conditions.Conclusion: We determined that AH-Ti/Sr90 possesses the best osteogenic property, long-term ion release capacity and osteointegration promotion ability. It has potential clinic application prospects.Keywords: titanium, alkali-heat treatment, magnetron sputtering, osteogenic differentiation, osteoclast inhibition

【 授权许可】

Unknown