Modified Titanium Surface-Mediated Effects on Human Bone Marrow Stromal Cell Response

Materials

Amol Chaudhari²  Joke Duyck²  Annabel Braem³  Jozef Vleugels³  Hervé Petite⁴  Delphine Logeart-Avramoglou⁴  Ignace Naert²  Johan A. Martens¹ 

[1] Center of Surface Chemistry and Catalysis, KU Leuven, Kasteelpark Arenberg 23 box 2461, Heverlee 3001, Belgium; E-Mail:;BIOMAT Research Cluster, Department of Oral Health Sciences and Prosthetic Dentistry, KU Leuven and University Hospitals Leuven, Kapucijnenvoer 7 box 7001, Leuven 3000, Belgium; E-Mails:;Department of Metallurgy and Materials Engineering (MTM), KU Leuven, Kasteelpark Arenberg 44 box 2450, Heverlee 3001, Belgium; E-Mails:;Laboratory of Bioengineering and Biomechanics for Bone Articulation, Faculty of Medicine, University Paris Diderot, 10 Avenue de Verdun, Paris 75010, France; E-Mails:

关键词: titanium;    surface coating;    human bone marrow stromal cells;    in vitro cytocompatibility;    osteogenic differentiation;    osseointegration;   

DOI  :  10.3390/ma6125533

来源: mdpi

【 摘 要 】

Surface modification of titanium implants is used to enhance osseointegration. The study objective was to evaluate five modified titanium surfaces in terms of cytocompatibility and pro-osteogenic/pro-angiogenic properties for human mesenchymal stromal cells: amorphous microporous silica (AMS), bone morphogenetic protein-2 immobilized on AMS (AMS + BMP), bio-active glass (BAG) and two titanium coatings with different porosity (T1; T2). Four surfaces served as controls: uncoated Ti (Ti), Ti functionalized with BMP-2 (Ti + BMP), Ti surface with a thickened titanium oxide layer (TiO₂) and a tissue culture polystyrene surface (TCPS). The proliferation of eGFP-fLuc (enhanced green fluorescence protein-firefly luciferase) transfected cells was tracked non-invasively by fluorescence microscopy and bio-luminescence imaging. The implant surface-mediated effects on cell differentiation potential was tracked by determination of osteogenic and angiogenic parameters [alkaline phosphatase (ALP); osteocalcin (OC); osteoprotegerin (OPG); vascular endothelial growth factor-A (VEGF-A)]. Unrestrained cell proliferation was observed on (un)functionalized Ti and AMS surfaces, whereas BAG and porous titanium coatings T1 and T2 did not support cell proliferation. An important pro-osteogenic and pro-angiogenic potential of the AMS + BMP surface was observed. In contrast, coating the Ti surface with BMP did not affect the osteogenic differentiation of the progenitor cells. A significantly slower BMP-2 release from AMS compared to Ti supports these findings. In the unfunctionalized state, Ti was found to be superior to AMS in terms of OPG and VEGF-A production. AMS is suggested to be a promising implant coating material for bioactive agents delivery.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.