Bhalin, an Essential Cytoskeleton-Associated Protein of Trypanosoma brucei Linking TbBILBO1 of the Flagellar Pocket Collar with the Hook Complex

Microorganisms

Brooke Morriswood¹  Katharina Havlicek²  Nicolas Landrein³  Denis Dacheux³  Anne Cayrel³  Christine E. Broster Reix³  Mélanie Bonhivers³  Célia Florimond³  Derrick R. Robinson³  Amélie Mailhé³  Corentin Agnero-Rigot³ 

[1] Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany;Max Perutz Labs, Vienna BioCenter, Dr. Bohr-Gasse 9, 1030 Vienna, Austria;Protist Parasite Cytoskeleton (ProParaCyto) Group, CNRS UMR 5234, Fundamental Microbiology and Pathogenicity, University of Bordeaux, 146 Rue Léo Saignat, 33076 Bordeaux, France;

关键词: trypanosoma;    flagellar pocket;    hook complex;    endocytosis;    cytoskeleton;    protozoan;   

DOI  :  10.3390/microorganisms9112334

来源: DOAJ

【 摘 要 】

Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The protein TbBILBO1 is located at the neck of the FP in a structure called the flagellar pocket collar (FPC) and is essential for biogenesis of the FPC and parasite survival. TbMORN1 is a protein that is present on a closely linked structure called the hook complex (HC) and is located anterior to and overlapping the collar. TbMORN1 is essential in the bloodstream form of T. brucei. We now describe the location and function of BHALIN, an essential, new FPC-HC protein. Methodology/Principal Findings: Here, we show that a newly characterised protein, BHALIN (BILBO1 Hook Associated LINker protein), is localised to both the FPC and HC and has a TbBILBO1 binding domain, which was confirmed in vitro. Knockdown of BHALIN by RNAi in the bloodstream form parasites led to cell death, indicating an essential role in cell viability. Conclusions/Significance: Our results demonstrate the essential role of a newly characterised hook complex protein, BHALIN, that influences flagellar pocket organisation and function in bloodstream form T. brucei parasites.

【 授权许可】

Unknown