Dynamic density shaping of photokinetic E. coli

eLife

Roberto Di Leonardo¹  Dario Dell'Arciprete²  Giacomo Frangipane²  Gaszton Vizsnyiczai²  Serena Petracchini³  Maria Lina Bernardini³  Silvio Bianchi⁴  Claudio Maggi⁴  Filippo Saglimbeni⁴ 

[1] Dipartimento di Biologia e Biotecnologie, Università di Roma "Sapienza", Roma, Italy;Dipartimento di Fisica, Università di Roma "Sapienza", Roma, Italy;Istituto Pasteur-Fondazione Cenci Bolognetti, Università di Roma "Sapienza", Roma, Italy;Soft and Living Matter Laboratory, Institute of Nanotechnology (NANOTEC-CNR), Roma, Italy;

关键词: active matter;    flagellar motility;    light response;   

DOI  :  10.7554/eLife.36608

来源: DOAJ

【 摘 要 】

Many motile microorganisms react to environmental light cues with a variety of motility responses guiding cells towards better conditions for survival and growth. The use of spatial light modulators could help to elucidate the mechanisms of photo-movements while, at the same time, providing an efficient strategy to achieve spatial and temporal control of cell concentration. Here we demonstrate that millions of bacteria, genetically modified to swim smoothly with a light controllable speed, can be arranged into complex and reconfigurable density patterns using a digital light projector. We show that a homogeneous sea of freely swimming bacteria can be made to morph between complex shapes. We model non-local effects arising from memory in light response and show how these can be mitigated by a feedback control strategy resulting in the detailed reproduction of grayscale density images.

【 授权许可】

Unknown