Developmental Biology | |
The t Complex Distorter 2 Candidate Gene, Dnahc8, Encodes at Least Two Testis-Specific Axonemal Dynein Heavy Chains That Differ Extensively at Their Amino and Carboxyl Termini | |
Olugbemiga Ogunkua1  Ling Hui1  John Fossella1  Sadhana A. Samant1  Stephen H. Pilder1  | |
[1] Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania, 19140 | |
关键词: t complex; t haplotype; dynein heavy chain; stem domain; motor domain; sperm tail; flagellar motility; flagellar morphogenesis; axoneme; fibrous sheath; | |
DOI : 10.1006/dbio.2002.0769 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
Homozygosityforthethaplotypealleleofthetestis-specificallyexpressedaxonemaldyneinheavychain(axDHC)gene,Dnahc8,hasbeenlinkedtomalesterilityresultingfromaberrantspermmotility.However,thenearabsenceofDnahc8expressionhasbeenassociatedwithmalesterilityresultingfromanearlybreakdowninspermflagellardevelopment.AlthoughaxDHCsareintegralparticipantsinflagellarmotility,aroleinflagellarmorphogenesishasneverbeenattributedtoamemberofthishighlyconservedgenefamily.TogainabetterunderstandingofthispresumednovelroleforDnahc8,wehavestudiedtheorganizationandexpressionoffull-lengthDnahc8+andDnahc8ttranscripts.Ourresultsdemonstratetheexistenceofatleasttwoalternativelyspliced,testis-specificDnahc8mRNAstranscribedfromboththe+andtalleles.AhighlyexpressedisoformencodesaproteinwithsignificanthomologynearlythroughouttotheγheavychainoftheChlamydomonasaxonemalouterarmdynein,whileamorepoorlyexpressedisoformcodesforaproteinwhosesequencedivergessignificantlyfromthatofotheraxDHCsatbothitsNandCtermini.WhileinsituhybridizationstudiesdemonstratethatbothmRNAspeciesaccumulateexclusivelyinmidtolatespermatocytes,eachisoformshowsspatialindependence.Additionalexperimentsdemonstratetheexistenceofatestis-expressedmRNAwithnosignificantopenreadingframe,aportionofwhichisantisensetothe5′-untranslatedregionofthehighlydivergentDnahc8isoform.ThecumulativedataimplythatDnahc8mayhaveacquiredfunctionalplasticityinthetestisthroughthetightlycontrolledexpressionofbothtypicalandunusualisoforms.
【 授权许可】
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