Differential Expression of CD45RO and CD45RA in Bovine T Cells

Cells

Akanksha Hada¹  Lei Li¹  Anmol Kandel¹  Zhengguo Xiao¹ 

[1] Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA;

关键词: cattle;    memory;    CD4+ T cells;    CD8+ T cells;    γδ T cells;    CD45RO;   

DOI  :  10.3390/cells11111844

来源: DOAJ

【 摘 要 】

Effective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulatory T cells with IFNγ and IL4 expression induced by a conventional method. To our surprise, 20% of cattle from an enclosed herd did not express CD45RO on T cells without any significant difference on CD45RA expression and IFNγ or IL4 induction. In CD45RO expressing cattle, CD45RA and CD45RO expressions excluded each other, with dominant CD45RO (>90%) expression on gamma delta (γδ) followed by CD4+ (60%) but significantly higher CD45RA expression on CD8+ T cells (about 80%). Importantly, more than 80% of CD45RO expressing CD4+ and CD8+ T cells failed to produce IFNγ and IL-4; however, within the cytokine inducing cells, CD4+ T cells highly expressed CD45RO but those within CD8+ T cells mostly expressed CD45RA. Hence, CD45RO is not ubiquitously expressed in cattle, and rather than with memory phenotype, CD45RA/RO expression are more associated with distinct T cell subtypes.

【 授权许可】

Unknown