Multimodal label-free imaging of living dermal equivalents including dermal papilla cells

Stem Cell Research & Therapy

Victoria G. Krut¹  Aleksandra V. Meleshina²  Daria S. Kuznetsova²  Marina A. Sirotkina²  Maria M. Lukina²  Varvara V. Dudenkova²  Elena V. Zagaynova²  Alena S. Bystrova²  Ekaterina P. Kalabusheva³  Olga S. Rogovaya³  Ekaterina A. Vorotelyak³  Andrey V. Vasiliev³ 

[1] Institute of Biology and Biomedicine, Nizhny Novgorod State University;Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy;Koltzov Institute of Developmental Biology, Russian Academy of Sciences;

关键词: Dermal equivalents;    Dermal papilla cells;    NAD(P)H;    CP OCT;    MPT;    FLIM;   

DOI  :  10.1186/s13287-018-0838-9

来源: DOAJ

【 摘 要 】

Abstract Background Despite the significant progress in the development of skin equivalents (SEs), the problem of noninvasively assessing the quality of the cell components and the collagen structure of living SEs both before and after transplantation remains. Undoubted preference is given to in vivo methods of noninvasive, label-free monitoring of the state of the SEs. Optical bioimaging methods, such as cross-polarization optical coherence tomography (CP OCT), multiphoton tomography (MPT), and fluorescence lifetime imaging microscopy (FLIM), present particular advantages for the visualization of such SEs. Methods In this study, we simultaneously applied several visualization techniques for skin model examination. We investigated the structure and quality of dermal equivalents containing dermal papilla (DP) cells and dermal fibroblasts (FBs) using CP OCT, MPT, and FLIM. Both the energy metabolism of the cell components and the structuring of the collagen fibrils were addressed. Results Based on the data from the fluorescence lifetimes and the contributions of protein-bound NAD(P)H, a bias toward oxidative metabolism was indicated, for the first time, in both the DP cells and FBs on day 14 of SE cultivation. The CP OCT and MPT data also indicated that both DP cells and FBs structured the collagen gel in a similar manner. Conclusion In this study, multimodal label-free imaging of the structure and quality of living dermal equivalents was implemented for the first time with the use CP OCT, MPT, and FLIM of NAD(P)H. Our data suggest that the combination of different imaging techniques provides an integrated approach to data acquisition regarding the structure and quality of dermal equivalents, minimizes the potential disadvantages of using a single method, and provides an ideal information profile for clinical and research applications.

【 授权许可】

Unknown