Optimization of Culture Conditions and Production of Bio-Fungicides from Trichoderma Species under Solid-State Fermentation Using Mathematical Modeling

Microorganisms

Negussie Megersa¹  Afrasa Mulatu²  Tesfaye Alemu²  Ramesh R. Vetukuri³ 

[1] Department of Chemistry, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia;Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia;Department of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, Sweden;

关键词: agro-industrial waste;    bioformulation;    response surface methodology;    viability;    wheat bran;   

DOI  :  10.3390/microorganisms9081675

来源: DOAJ

【 摘 要 】

Agro-industrial wastes suitable for economical and high mass production of novel Trichoderma species under solid-state fermentation were identified by optimizing the culture conditions using a mathematical model and evaluating the viability of the formulated bio-product. Fourteen inexpensive, locally available, organic substrates and cereals were examined using a one-factor-at-a-time experiment. The fungus colonized nearly all substrates after 21 days of incubation, although the degree of colonization and conidiation varied among the substrates. A mixture of wheat bran and white rice (2:1 w/w) was found to support maximum growth of T. asperellum AU131 (3.2 × 10⁷ spores/g dry substrate) and T. longibrachiatum AU158 (3.5 × 10⁷ spores/g dry substrate). Using a fractional factorial design, the most significant growth factors influencing biomass production were found to be temperature, moisture content, inoculum concentration, and incubation period (p ≤ 0.05). Analysis of variance of a Box–Behnken design showed that the regression model was highly significant (p ≤ 0.05) with F-values of 10.38 (P = 0.0027, T. asperellum AU131) and 12.01 (p < 0.0017, T. longibrachiatum AU158). Under optimal conditions, maximum conidia yield of log₁₀ (8.6) (T. asperellum AU131) and log₁₀(9.18) (T. longibrachiatum) were obtained. For wettable powder Trichoderma species formulations, it was possible to maintain conidial viability at room temperature (25 °C) for eight months at concentrations above 10⁶ CFU/g.

【 授权许可】

Unknown