Exploring Serum NMR-Based Metabolomic Fingerprint of Colorectal Cancer Patients: Effects of Surgery and Possible Associations with Cancer Relapse

Applied Sciences

Chiara Biagioni¹  Matteo Benelli¹  Dario Romagnoli¹  Laura Biganzoli²  Samantha Di Donato²  Luca Malorni²  Francesca Del Monte²  Vanessa Calamai²  Annamaria Parnofiello²  Elena Mori²  Maddalena Baraghini³  Alessia Garzi³  Stefano Cantafio³  Leonardo Tenori⁴  Claudio Luchinat⁴  Alessia Vignoli⁴  Ilenia Migliaccio⁵ 

[1] Bioinformatics Unit, Medical Oncology Department, New Hospital of Prato-S. Stefano, 59100 Prato, Italy;Department of Medical Oncology, New Hospital of Prato-S. Stefano, 59100 Prato, Italy;Department of Surgery, New Hospital of Prato-S. Stefano, 59100 Prato, Italy;Magnetic Resonance Center (CERM) and Department of Chemistry “Ugo Schiff”, University of Florence, 50019 Sesto Fiorentino, Italy;“Sandro Pitigliani” Translational Research Unit, New Hospital of Prato-S. Stefano, 59100 Prato, Italy;

关键词: metabolomics;    colorectal cancer;    nuclear magnetic resonance;    surgery;    relapse;   

DOI  :  10.3390/app112311120

来源: DOAJ

【 摘 要 】

Background: Colorectal cancer (CRC) is the fourth most commonly diagnosed and third most deadly cancer worldwide. Surgery is the main treatment option for early disease; however, a relevant proportion of CRC patients relapse. Here, variations among preoperative and postoperative serum metabolomic fingerprint of CRC patients were studied, and possible associations between metabolic variations and cancer relapse were explored. Methods: A total of 41 patients with stage I-III CRC, planned for radical resection, were enrolled. Serum samples, collected preoperatively (t0) and 4–6 weeks after surgery before the start of any treatment (t1), were analyzed via NMR spectroscopy. NMR data were analyzed using multivariate and univariate statistical approaches. Results: Serum metabolomic fingerprints show differential clustering between t0 and t1 (82–85% accuracy). Pyruvate, HDL-related parameters, acetone, and 3-hydroxybutyrate appear to be the major players in this discrimination. Eight out of the 41 CRC patients enrolled developed cancer relapse. Postoperative, relapsed patients show an increase of pyruvate and HDL-related parameters, and a decrease of Apo-A1 Apo-B100 ratio and VLDL-related parameters. Conclusions: Surgery significantly alters the metabolomic fingerprint of CRC patients. Some metabolic changes seem to be associated with the development of cancer relapse. These data, if validated in a larger cohort, open new possibilities for risk stratification in patients with early-stage CRC.

【 授权许可】

Unknown