Protective Effect of Anthocyanin on Neurovascular Unit in Cerebral Ischemia/Reperfusion Injury in Rats

Frontiers in Neuroscience

Tianjie Yuan¹  Yuhua Li¹  Zihao Pan¹  Yang Pan¹  Tuo Ji¹  Guoliang Dai²  Mengdi Cui³ 

[1] College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China;Department of Clinical Pharmacology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China;Thyroid and Breast Surgery, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China;

关键词: cerebral ischemia/reperfusion injury;    neurovascular unit;    anthocyanin;    apoptosis;    inflammation;   

DOI  :  10.3389/fnins.2018.00947

来源: DOAJ

【 摘 要 】

Treating cerebral ischemia continues to be a clinical challenge. Studies have shown that the neurovascular unit (NVU), as the central structural basis, plays a key role in cerebral ischemia. Here, we report that anthocyanin, a safe and natural antioxidant, could inhibit apoptosis and inflammation to protect NVU in rats impaired by middle cerebral artery occlusion/reperfusion (MCAO/R). Administration of anthocyanin significantly reduced infarct volume and neurological scores in MCAO/R rats. Anthocyanin could also markedly ameliorate cerebral edema and reduce the concentration of Evans blue (EB) by inhibiting MMP-9. Moreover, anthocyanin alleviated apoptotic injury resulting from MCAO/R through the regulation of Bcl-2 family proteins. The levels of inflammation-related molecules including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), which were over-expressed with MCAO/R, were decreased by anthocyanin. In addition, Nuclear factor-kappa B (NF-κB) and the NLRP3 inflammasome pathway might be involved in the anti-inflammatory effect of anthocyanin. In conclusion, anthocyanin could protect the NVU through multiple pathways, and play a protective role in cerebral ischemia/reperfusion injury.

【 授权许可】

Unknown