Bioreactor Systems for Human Bone Tissue Engineering

Processes

Martina Sladkova¹  Giuseppe Maria de Peppo¹ 

[1] The New York Stem Cell Foundation Research Institute, 1995 Broadway,New York, NY 10023, USA;

关键词: human cells;    stem cells;    biomaterial scaffolds;    spinner flasks;    rotating wall vessels;    flow perfusion bioreactors;    compression systems;    osteogenic differentiation;    bone engineering;    bone defects;    skeletal reconstructions;   

DOI  :  10.3390/pr2020494

来源: DOAJ

【 摘 要 】

Critical size skeletal defects resulting from trauma and pathological disorders still remain a major clinical problem worldwide. Bone engineering aims at generating unlimited amounts of viable tissue substitutes by interfacing osteocompetent cells of different origin and developmental stage with compliant biomaterial scaffolds, and culture the cell/scaffold constructs under proper culture conditions in bioreactor systems. Bioreactors help supporting efficient nutrition of cultured cells and allow the controlled provision of biochemical and biophysical stimuli required for functional regeneration and production of clinically relevant bone grafts. In this review, the authors report the advances in the development of bone tissue substitutes using human cells and bioreactor systems. Principal types of bioreactors are reviewed, including rotating wall vessels, spinner flasks, direct and indirect flow perfusion bioreactors, as well as compression systems. Specifically, the review deals with: (i) key elements of bioreactor design; (ii) range of values ofstress imparted to cells and physiological relevance; (iii) maximal volume of engineered bone substitutes cultured in different bioreactors; and (iv) experimental outcomes and perspectives for future clinical translation.

【 授权许可】

Unknown