Neuroprotective effect of omega‐3 fatty acids on spinal cord injury induced rats

Brain and Behavior

Chong Chen¹  Haining Tan¹  Jianxiong Shen¹  Peiyu Sun¹  Jiaqi Bi¹  Fan Feng² 

[1] Department of Orthopedic Surgery Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China;Department of Orthopedics Surgery, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China;

关键词: apoptosis;    inflammation;    omega‐3 fatty acid;    oxidative stress;    rat;   

DOI  :  10.1002/brb3.1339

来源: DOAJ

【 摘 要 】

Abstract Introduction In this study, the effects of omega‐3 fatty acids were examined in a rat model of spinal cord injury. Methods The rats were classified into sham, control, spinal cord injury plus 50 mg/kg Omega‐3 fatty acids and spinal cord injury plus 100 mg/kg Omega‐3 fatty acids. The levels of oxidative, apoptotic, and inflammatory markers were examined in each of these groups. Results Altered lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (Gpx), and catalase were normalized. Omega‐3 fatty acid supplementation decreased tumor necrosis factor‐alpha (TNF‐α) and interleukin‐6 (IL‐6) levels by >50%. TNF‐α and IL‐6 mRNA expression were reduced. Caspase‐3, p53, bax, and pro‐NGF mRNA expression levels were increased by 1.3‐, 1.4‐, 1.2‐, and 0.9‐fold, respectively, whereas bcl‐2 mRNA expression was decreased by 0.77‐fold in control rats. Omega‐3 fatty acid supplementation decreased p53, caspase‐3, bax, and pro‐NGF mRNA expression by >40%, while the level of bcl‐2 mRNA expression was increased by 286.9%. Omega‐3 fatty acid supplementation decreased caspase‐3 and p53 protein expression by >30%. Conclusion Taken together, our results suggested that omega‐3 fatty acid supplementation reduced oxidative stress, apoptosis, and the levels of inflammatory markers in ischemia‐reperfusion‐induced rats.

【 授权许可】

Unknown