Combined effects of PEGylation and particle size on uptake of PLGA particles by macrophage cells

Nanomedicine Journal

Bahman Khameneh¹  Tina Moayedian²  Fatemeh Mosaffa³  Mohsen Tafaghodi⁴ 

[1] Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

[2] Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran;School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

[3] Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

[4] Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;

关键词: PLGA particles;    PEGylation;    Phagocytosis;    Size;   

DOI  :  10.7508/nmj.2015.04.008

来源: DOAJ

【 摘 要 】

Objective: At the present study, relationship between phagocytosis of PLGA particles and combined effects of particle size and surface PEGylation was investigated. Materials and Methods: Microspheres and nanospheres (3500 nm and 700 nm) were prepared from three types of PLGA polymers (non-PEGylated and PEGylation percents of 9% and 15%). These particles were prepared by solvent evaporation method. All particles were labeled with FITC-Albumin. Interaction of particles with J744.A.1 mouse macrophage cells, was evaluated in the absence or presence of 7% of the serum by flowcytometry method. Results: The study revealed more phagocytosis of nanospheres. In the presence of the serum, PEGylated particles were phagocytosed less than non-PEGylated particles. For nanospheres, this difference was significant (P

【 授权许可】

Unknown