Study of hepatocellular function in the murine model following hepatic artery selective clamping

Acta Cirúrgica Brasileira

Ricardo Martins¹  Dulce Cardoso²  Emir Hoti³  Bárbara Oliveiros⁴  Maria Filomena Botelho⁴  Ana Cristina Gonçalves⁴  José Guilherme Tralhão⁴  Mafalda Laranjo⁴  Ana Bela Sarmento-Ribeiro⁴  Ana Margarida Abrantes⁴  Francisco Castro-Sousa⁴ 

[1] ;Centro Hospitalar e Universitário de Coimbra;Saint Vincents' University Hospital;Universidade de Coimbra;

关键词: Liver;    Hepatic Artery;    Constriction;    Radionuclide Imaging;    Rats;   

DOI  :  10.1590/S0102-86502013000900006

来源: DOAJ

【 摘 要 】

PURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability. RESULTS: A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species. CONCLUSIONS: The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function.

【 授权许可】

Unknown