Study of hepatocellular function in the murine model following hepatic artery selective clamping

Acta Cirurgica Brasileira

José Guilherme Tralhão²  Ana Margarida Abrantes¹  Ana Cristina Gonçalves¹  Emir Hoti¹  Mafalda Laranjo¹  Ricardo Martins¹  Bárbara Oliveiros¹  Dulce Cardoso¹  Ana Bela Sarmento-ribeiro¹  Maria Filomena Botelho¹  Francisco Castro-sousa¹ 

[1] ,University of Coimbra Faculty of Medicine Surgery Department,Portugal

关键词: Liver;    Hepatic Artery;    Constriction;    Radionuclide Imaging;    Rats;   

DOI  :  10.1590/S0102-86502013000900006

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability. RESULTS: A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species. CONCLUSIONS: The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License