VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

Biology

IvanV. Kulakovskiy¹  Mikhail Eldarov²  Alexey Fedorov²  Ludmila Trilisenko³  Alexander Tomashevsky³  Tatiana Kulakovskaya³  Ekaterina Kulakovskaya³ 

[1] Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia;Federal Scientific Center for Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, Russian Academy of Sciences, Leninsky prosp. 33-2, 119071 Moscow, Russia;Federal Scientific Center, Pushchino Research Center for Biology of the Russian Academy of Sciences, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290 Pushchino, Russia;

关键词: inorganic polyphosphate;    yeast;    VTC4;    oxidative and heavy metal stress;    DDR2;    PHO84;   

DOI  :  10.3390/biology10060487

来源: DOAJ

【 摘 要 】

Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg²⁺-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast.

【 授权许可】

Unknown