Calcium Polyphosphate Nanoparticles Act as an Effective Inorganic Phosphate Source during Osteogenic Differentiation of Human Mesenchymal Stem Cells

International Journal of Molecular Sciences

Luan Phelipe Hatt¹  MartinJames Stoddart¹  Keith Thompson¹  AngelaRita Armiento¹  WernerE. G. Müller² 

[1] AO Research Institute Davos, 7270 Davos Platz, Switzerland;Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany;

关键词: osteogenic differentiation;    mesenchymal stem cells;    β-glycerolphosphate;    inorganic polyphosphate;    ca-polyphosphate nanoparticles;   

DOI  :  10.3390/ijms20225801

来源: DOAJ

【 摘 要 】

The ability of bone-marrow-derived mesenchymal stem/stromal cells (BM-MSCs) to differentiate into osteoblasts makes them the ideal candidate for cell-based therapies targeting bone-diseases. Polyphosphate (polyP) is increasingly being studied as a potential inorganic source of phosphate for extracellular matrix mineralisation. The aim of this study is to investigate whether polyP can effectively be used as a phosphate source during the in vitro osteogenic differentiation of human BM-MSCs. Human BM-MSCs are cultivated under osteogenic conditions for 28 days with phosphate provided in the form of organic β-glycerolphosphate (BGP) or calcium-polyP nanoparticles (polyP-NP). Mineralisation is demonstrated using Alizarin red staining, cellular ATP content, and free phosphate levels are measured in both the cells and the medium. The effects of BGP or polyP-NP on alkaline phosphatase (ALP) activity and gene expression of a range of osteogenic-related markers are also assessed. PolyP-NP supplementation displays comparable effects to the classical BGP-containing osteogenic media in terms of mineralisation, ALP activity and expression of osteogenesis-associated genes. This study shows that polyP-NP act as an effective source of phosphate during mineralisation of BM-MSC. These results open new possibilities with BM-MSC-based approaches for bone repair to be achieved through doping of conventional biomaterials with polyP-NP.

【 授权许可】

Unknown