Self-Synchronized Oscillatory Metabolism of Clostridium pasteurianum in Continuous Culture

Processes

Lars Rehmann¹  ErinE Johnson² 

[1] Biochemical Engineering, The University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada;;Department of Chemical &

关键词: butanol;    glycerol;    clostridium;    pasteurianum;    synchronized;    oscillatory;    metabolism;    redox;    cstr;   

DOI  :  10.3390/pr8020137

来源: DOAJ

【 摘 要 】

By monitoring the real-time gas production (CO₂ and H₂) and redox potential at high sampling frequency in continuous culture of Clostridium pasteurianum on glycerol as sole carbohydrate, the self-synchronized oscillatory metabolism was revealed and studied. The oscillations in CO₂ and H₂ production were in sync with each other and with both redox potential and glycerol in the continuous stirred tank reactor (CSTR). There is strong evidence that the mechanism for this is in the regulation of the oxidative pathway of glycerol metabolism, including glycolysis, and points toward complex, concerted cycles of enzyme inhibition and activation by pathway intermediates and/or redox equivalents. The importance of understanding such an “oscillatory metabolism” is for developing a stable and highly productive industrial fermentation process for butanol production, as unstable oscillations are unproductive. It is shown that the oscillatory metabolism can be eradicated and reinstated and that the period of oscillations can be altered by modification of the operating parameters. Synchronized oscillatory metabolism impacted the product profile such that it lowered the selectivity for butanol and increased the selectivity for ethanol. This elucidates a possible cause for the variability in the product profile of C. pasteurianum that has been reported in many previous studies.

【 授权许可】

Unknown