A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains

Molecules

Hao Pang¹  Lihua Lin¹  Hongchi Tang¹  Yuan Guo¹  Liqin Du²  Zhikai Zhang²  Yutuo Wei²  Ribo Huang²  Shaowei Zeng² 

[1] National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, China;

关键词: sucrose transporter;    esculin;    fluorescence assay;    clostridium;   

DOI  :  10.3390/molecules24193495

来源: DOAJ

【 摘 要 】

A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as ¹⁴C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium, and a fluorescence assay based on esculin was used for the analysis of sucrose transport in Clostridium strains. This showed that the heterologously expressed potato sucrose transporter is functional in Clostridium. Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in Clostridium strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in Clostridium.

【 授权许可】

Unknown