Heliyon | |
Mancozeb impairs mitochondrial and bioenergetic activity in Drosophila melanogaster | |
Illana Kemmerich Martins1  Karen Kich Gomes2  Giulianna Echeverria Macedo3  Marina Prigol3  Miriane Acosta Saraiva3  Jeferson Luis Franco3  Thais Posser3  Nathane Rosa Rodrigues3  Nelson Rodrigues de Carvalho3  Patrícia de Brum Vieira3  Cynthia Camila Ziech3  | |
[1] Instituto Federal Farroupilha, Campus Santo Ângelo, 98806700, RS, Brazil;Laboratório de Avaliações Farmacológicas e Toxicológicas aplicadas às Moléculas Bioativas – Unipampa, Universidade Federal do Pampa - Campus Itaqui, Itaqui, RS, 97650-000, Brazil;Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000, São Gabriel, RS, Brazil; | |
关键词: Respiration; ACeCl-1; Mitochondria; Mancozeb; Drosophila melanogaster; Glucose; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Mancozeb (MZ) is a broad-spectrum fungicide used worldwide in several crops. Neurological disorders in humans and animals have been associated with exposure to this compound by mechanisms still not fully understood. Drosophila melanogaster represents a reliable model in toxicological studies, presenting genetic and biochemical similarities with mammals. In this study, D. melanogaster flies were exposed for 15 days to MZ through the food (5 and 10 mg/mL). After that period, the efficiency of mitochondrial respiration complexes and metabolic markers were analyzed and evaluated. Flies presented weight loss, lower glucose, trehalose, and glycogen levels, and augmented levels of triglycerides concerning control (non-treated group). Acetyl-CoA Synthetase (ACeCS-1) and Acyl-Coenzyme Synthetase (ACSL1) contents were unchanged by MZ treatment. Mitochondrial respiration of flies was targeted by MZ treatment, evidenced by a decrease in oxygen consumption and bioenergetics rate and inhibition in mitochondrial complexes I/II. These results suppose that an impairment in mitochondrial respiration jointly with reduced levels of energetic substrates might be a mechanism involved in MZ deleterious effects, possibly by the limitation of ATP's availability, necessary for essential cellular processes.
【 授权许可】
Unknown