Quantification of Iron Release from Native Ferritin and Magnetoferritin Induced by Vitamins B2 and C

International Journal of Molecular Sciences

Alzbeta Trancikova¹  Michal Pokusa¹  Oliver Strbak¹  Jan Gombos²  Martina Kmetova²  Peter Kopcansky³  Lucia Balejcikova⁴ 

[1] Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, Slovakia;Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, Slovakia;Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, Slovakia;Institute of Hydrology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovakia;

关键词: ferritin;    magnetoferritin;    mineral core;    reduction;    ascorbic acid;    riboflavin;   

DOI  :  10.3390/ijms21176332

来源: DOAJ

【 摘 要 】

Various pathological processes in humans are associated with biogenic iron accumulation and the mineralization of iron oxide nanoparticles, especially magnetite. Ferritin has been proposed as a precursor to pathological magnetite mineralization. This study quantifies spectroscopically the release of ferrous ions from native ferritin and magnetoferritin as a model system for pathological ferritin in the presence of potent natural reducing agents (vitamins C and B₂) over time. Ferrous cations are required for the transformation of ferrihydrite (physiological) into a magnetite (pathological) mineral core and are considered toxic at elevated levels. The study shows a significant difference in the reduction and iron release from native ferritin compared to magnetoferritin for both vitamins. The amount of reduced iron formed from a magnetoferritin mineral core is two to five times higher than from native ferritin. Surprisingly, increasing the concentration of the reducing agent affects only iron release from native ferritin. Magnetoferritin cores with different loading factors seem to be insensitive to different concentrations of vitamins. An alternative hypothesis of human tissue magnetite mineralization and the process of iron-induced pathology is proposed. The results could contribute to evidence of the molecular mechanisms of various iron-related pathologies, including neurodegeneration.

【 授权许可】

Unknown