期刊论文详细信息
Journal of Functional Foods
Probiotic bacteria induced improvement of the mucosal integrity of enterocyte-like Caco-2 cells after exposure to Salmonella enteritidis 857
Peter C.J. Tooten1  Joshua J. Malago2  Jos F.J.G. Koninkx3 
[1] Corresponding author: Tel.: +31 30 2534307;fax: +31 30 2516853.;Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands;
关键词: Probiotic bacteria;    Salmonella enteritidis 857;    Trans-epithelial electrical resistance;    Caco-2 cells;    Heat shock proteins;   
DOI  :  
来源: DOAJ
【 摘 要 】

Probiotics protect the intestinal epithelium. Whether their protection against Salmonella is mediated via stabilizing the barrier integrity was examined here. The intestinal barrier integrity was assessed by measuring the trans-epithelial electrical resistance (TER) of Caco-2 cell monolayer. Cells were exposed for 1 h to Lactobacillus casei W56, Lactobacillus salivarius W24, Lactobacillus acidophilus W70, Lactococcus lactis W58, Bifidobacterium infantis W52 or Bifidobacterium bifidum W23 at 200 bacteria/cell or to Salmonella enteritidis 857 at 1–200 bacteria/cell or to a combination of either of the probiotic and Salmonella both at 200 bacteria/cell. Heat shock protein (Hsp) 70 was assessed by Western blotting. Probiotics induced a significant increase while Salmonella decreased the TER. The latter was attenuated by probiotics but without full recovery. In addition, probiotics induced over-expression of Hsp70. It may be concluded that probiotics stabilise the intestinal integrity and impede Salmonella infection via, at least in part, production of Hsp70.

【 授权许可】

Unknown   

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