A Novel Strategy for Efficient Agaro-Oligosaccharide Production Based on the Enzymatic Degradation of Crude Agarose in Flammeovirga pacifica WPAGA1

Frontiers in Microbiology

Runying Zeng¹  Du Zhu²  Boliang Gao³  Li Li³  Min Jin³  Wu Qu³  Hui Wu⁴ 

[1] Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China;Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, China;State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China;

关键词: Flammeovirga pacifica WPAGA1;    metabolic pathway;    crude agarose;    agaro-oligosaccharides;    biosynthesis;   

DOI  :  10.3389/fmicb.2019.01231

来源: DOAJ

【 摘 要 】

To avoid conflict between biofuel and food resource production, marine macroalgae (main algal polysaccharides) have been suggested as potent feedstock for biofuel production. Flammeovirga pacifica WPAGA1, a typical marine polysaccharide-degrading bacterium, can utilize crude agarose as the sole carbon source. Transcriptomic analysis was performed to further investigate the metabolic pathway of environmental-friendly utilization of crude agarose in F. pacifica WPAGA1. All these enzymes were overexpressed in Escherichia coli BL21(DE3), and the purified enzymes were characterized in vitro. As a result, the pathway of crude agarose which is desulfurized and hydrolyzed by enzymes to produce fermentable sugar is clear. Interestingly, sole neoagarobiose (~450 mg/L) was produced from crude agarose as a feedstock using engineered E. coli BL21(DE3). This study firstly reveals the metabolic pathway of crude agarose in strain WPAGA1 and establishes a novel and environmental-friendly strategy for neoagarobiose production using crude agarose as cost-effective and non-food-based feedstock.

【 授权许可】

Unknown