Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

Revista Brasileira de Farmacognosia

Diogo R. B. Ducatti²  Franciely G. Colodi²  Alan G. Gonçalves¹  M. Eugênia R. Duarte²  Miguel D. Noseda² 

[1] ,Universidade Federal do Paraná Departamento de Bioquímica e Biologia Molecular ,Brazil

关键词: agaro-oligosaccharides;    carra-oligosaccharides;    partial acid hydrolysis;    3;    6-anhydro-galactose;    agarose;    kappa-carrageenan;   

DOI  :  10.1590/S0102-695X2011005000080

来源: SciELO

【 摘 要 】

Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.

【 授权许可】

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