Longitudinal alterations of the gut mycobiota and microbiota on COVID-19 severity

BMC Infectious Diseases

Hiroya Oki¹  Saori Amiya²  Reina Hara²  Yoshito Takeda²  Takahiro Kawasaki²  Yoshimi Noda²  Yuichi Adachi²  Haruhiko Hirata²  Eri Oguro-Igashira²  Takayuki Niitsu²  Kiyoshi Takeda³  Yuichi Maeda³  Shota Okamoto³  Hiroshi Kida⁴  Takanori Matsuki⁴  Kiyoharu Fukushima⁵  Kentaro Tanaka⁵  Atsushi Kumanogoh⁵  Daisuke Motooka⁵  Shota Nakamura⁵ 

[1] Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University;Department of Respiratory Medicine, Clinical Immunology, Graduate School of Medicine, Osaka University;Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University;National Hospital Organization Osaka Toneyama Medical Center;WPI Immunology Frontier Research Center, Osaka University;

关键词: COVID-19;    Mycobiota;    Microbiota;    Gut;    Candida;    Enterococcus;   

DOI  :  10.1186/s12879-022-07358-7

来源: DOAJ

【 摘 要 】

Abstract Background The impact of SARS-CoV-2 infection on the gut fungal (mycobiota) and bacterial (microbiota) communities has been elucidated individually. This study analyzed both gut mycobiota and microbiota and their correlation in the COVID-19 patients with severe and mild conditions and follow-up to monitor their alterations after recovery. Methods We analyzed the gut mycobiota and microbiota by bacterial 16S and fungal ITS1 metagenomic sequencing of 40 severe patients, 38 mild patients, and 30 healthy individuals and reanalyzed those of 10 patients with severe COVID-19 approximately 6 months after discharge. Results The mycobiota of the severe and mild groups showed lower diversity than the healthy group, and in some, characteristic patterns dominated by a single fungal species, Candida albicans, were detected. Lower microbial diversity in the severe group was observed, but no differences in its diversity or community structure were detected between the mild and healthy groups. The microbiota of the severe group was characterized by an increase in Enterococcus and Lactobacillus, and a decrease in Faecalibacterium and Bacteroides. The abundance of Candida was positively correlated with that of Enterococcus in patients with COVID-19. After the recovery of severe patients, alteration of the microbiota remained, but the mycobiota recovered its diversity comparable to that of mild and healthy groups. Conclusion In mild cases, the microbiota is stable during SARS-CoV-2 infection, but in severe cases, alterations persist for 6 months after recovery.

【 授权许可】

Unknown