Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release

Microbial Cell

Alexandre Légiot¹  Mohamed Kaabouni¹  Nadine Camougrand¹  Stéphen Manon¹  Claire Céré¹  Thibaud Dupoiron¹ 

[1] Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS & Université de Bordeaux, Campus Carreire, CS61390, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France.;

关键词: Bax;    apoptosis;    mitochondria associated membranes;    mitochondria;    cytochrome c;    ERMES;    density gradients;    Saccharomyces cerevisiae;   

DOI  :  10.15698/mic2019.05.678

来源: DOAJ

【 摘 要 】

The distribution of the pro-apoptotic protein Bax in the outer mitochondrial membrane (OMM) is a central point of regulation of apoptosis. It is now widely recognized that parts of the endoplasmic reticulum (ER) are closely associated to the OMM, and are actively involved in different signaling processes. We addressed a possible role of these domains, called Mitochondria-Associated Membranes (MAMs) in Bax localization and function, by expressing the human protein in a yeast mutant deleted of MDM34, a ERMES (ER-Mitochondria Encounter Structure) component. By affecting MAMs stability, the deletion of MDM34 altered Bax mitochondrial localization, and decreased its capacity to release cytochrome c. Furthermore, the deletion of MDM34 decreased the size of an incompletely released, MAMs-associated pool of cytochrome c.

【 授权许可】

Unknown